Rabbit Interleukin 4,IL-4 ELISA Kit
Product Details
| Target Name |
interleukin 4 |
| Alternative Names |
IL4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit |
| Abbreviation |
IL4 |
| Species |
Oryctolagus cuniculus (Rabbit) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.625 pg/mL-40 pg/mL |
| Sensitivity |
0.156 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
86 |
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| Range % |
80-92 |
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| 1:2 |
Average % |
100 |
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| Range % |
91-105 |
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| 1:4 |
Average % |
97 |
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| Range % |
92-105 |
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| 1:8 |
Average % |
94 |
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| Range % |
86-98 |
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| Recovery |
| The recovery of rabbit IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
93 |
89-98 |
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| EDTA plasma (n=4) |
95 |
90-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 40 |
2.804 |
2.786 |
2.795 |
2.706 |
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| 20 |
2.280 |
2.176 |
2.228 |
2.139 |
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| 10 |
1.544 |
1.443 |
1.494 |
1.405 |
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| 5 |
0.899 |
0.876 |
0.888 |
0.799 |
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| 2.5 |
0.443 |
0.423 |
0.433 |
0.344 |
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| 1.25 |
0.230 |
0.226 |
0.228 |
0.139 |
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| 0.625 |
0.154 |
0.149 |
0.152 |
0.063 |
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| 0 |
0.090 |
0.088 |
0.089 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
