Rabbit Interleukin 6,IL-6 ELISA Kit
Product Details
| Target Name |
interleukin 6 (interferon, beta 2) |
| Alternative Names |
IL6Interleukin-6 ELISA Kit; IL-6 ELISA Kit |
| Abbreviation |
IL6 |
| Species |
Oryctolagus cuniculus (Rabbit) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
15.6 pg/mL-1000 pg/mL |
| Sensitivity |
3.9 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
88 |
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| Range % |
80-92 |
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| 1:2 |
Average % |
99 |
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| Range % |
91-105 |
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| 1:4 |
Average % |
101 |
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| Range % |
92-110 |
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| 1:8 |
Average % |
93 |
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| Range % |
86-98 |
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| Recovery |
| The recovery of rabbit IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
93 |
89-98 |
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| EDTA plasma (n=4) |
96 |
90-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.840 |
2.785 |
2.813 |
2.710 |
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| 500 |
2.316 |
2.223 |
2.270 |
2.167 |
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| 250 |
1.580 |
1.567 |
1.574 |
1.471 |
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| 125 |
0.935 |
0.874 |
0.905 |
0.802 |
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| 62.5 |
0.479 |
0.462 |
0.471 |
0.368 |
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| 31.2 |
0.266 |
0.254 |
0.260 |
0.157 |
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| 15.6 |
0.170 |
0.158 |
0.164 |
0.061 |
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| 0 |
0.107 |
0.099 |
0.103 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
