Rabbit Interleukin 8,IL-8 ELISA Kit
Product Details
| Target Name |
interleukin 8 |
| Alternative Names |
CXCL8 ELISA Kit; IL8Interleukin-8 ELISA Kit; IL-8 ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit; Neutrophil attractant/activation protein 1 ELISA Kit; NAP-1 ELISA Kit; Permeability factor 1 ELISA Kit; PF1 ELISA Kit; rPF1 ELISA Kit |
| Abbreviation |
IL8 |
| Species |
Oryctolagus cuniculus (Rabbit) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
23.5 pg/mL-1500 pg/mL |
| Sensitivity |
5.8 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
101 |
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| Range % |
94-105 |
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| 1:2 |
Average % |
97 |
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| Range % |
91-102 |
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| 1:4 |
Average % |
89 |
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| Range % |
85-94 |
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| 1:8 |
Average % |
90 |
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| Range % |
86-98 |
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| Recovery |
| The recovery of rabbit IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
93 |
87-96 |
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| EDTA plasma (n=4) |
92 |
89-94 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1500 |
2.390 |
2.345 |
2.368 |
2.285 |
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| 750 |
1.587 |
1.476 |
1.532 |
1.449 |
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| 375 |
0.814 |
0.778 |
0.796 |
0.713 |
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| 187.5 |
0.409 |
0.389 |
0.399 |
0.316 |
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| 94 |
0.276 |
0.264 |
0.270 |
0.187 |
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| 47 |
0.182 |
0.172 |
0.177 |
0.094 |
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| 23.5 |
0.134 |
0.131 |
0.133 |
0.050 |
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| 0 |
0.085 |
0.081 |
0.083 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
