Rabbit Krebs yon den Lundgen-6 (KL-6) ELISA Kit
Product Details
| Target Name |
Krebs yon den Lundgen-6 (KL-6) |
| Abbreviation |
KL-6 |
| Uniprot No. |
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| Species |
Oryctolagus cuniculus (Rabbit) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
50 IU/mL-800 IU/mL |
| Sensitivity |
25 IU/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others |
| Assay Principle |
quantitative |
| Measurement |
Competitive |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit KL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
87 |
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| Range % |
82-93 |
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| 1:2 |
Average % |
94 |
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| Range % |
89-99 |
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| 1:4 |
Average % |
92 |
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| Range % |
87-96 |
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| 1:8 |
Average % |
95 |
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| Range % |
90-100 |
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| Recovery |
| The recovery of rabbit KL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
91-99 |
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| EDTA plasma (n=4) |
89 |
87-93 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| IU/ml |
OD1 |
OD2 |
Average |
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| 800 |
0.191 |
0.198 |
0.195 |
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| 400 |
0.351 |
0.337 |
0.344 |
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| 200 |
0.614 |
0.627 |
0.621 |
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| 100 |
1.154 |
1.132 |
1.143 |
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| 50 |
2.330 |
2.224 |
2.277 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
