Rabbit Platelet-Derived Growth Factor AB(PDGF-AB)ELISA Kit
Product Details
| Target Name |
Platelet-Derived Growth Factor AB(PDGF-AB) |
| Abbreviation |
PDGF-AB |
| Species |
Oryctolagus cuniculus (Rabbit) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
1.56 ng/mL-100 ng/mL |
| Sensitivity |
0.39 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit PDGF-AB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
86 |
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| Range % |
82-92 |
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| 1:2 |
Average % |
87 |
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| Range % |
83-95 |
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| 1:4 |
Average % |
94 |
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| Range % |
90-98 |
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| 1:8 |
Average % |
101 |
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| Range % |
96-105 |
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| Recovery |
| The recovery of rabbit PDGF-AB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
87 |
83-93 |
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| EDTA plasma (n=4) |
93 |
89-97 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 100 |
2.846 |
2.734 |
2.790 |
2.696 |
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| 50 |
2.147 |
2.035 |
2.091 |
1.997 |
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| 25 |
1.293 |
1.248 |
1.271 |
1.177 |
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| 12.5 |
0.688 |
0.677 |
0.683 |
0.589 |
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| 6.25 |
0.412 |
0.424 |
0.418 |
0.324 |
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| 3.12 |
0.278 |
0.289 |
0.284 |
0.190 |
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| 1.56 |
0.151 |
0.153 |
0.152 |
0.058 |
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| 0 |
0.093 |
0.094 |
0.094 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
