Rabbit terminal complement complex C5b-9 (TCC C5b-9)ELISA Kit
Product Details
| Target Name |
terminal complement complex C5b-9 (TCC C5b-9) |
| Abbreviation |
TCC C5b-9 |
| Species |
Oryctolagus cuniculus (Rabbit) |
| Sample Types |
serum, plasma |
| Detection Range |
7.8 ng/mL-500 ng/mL |
| Sensitivity |
1.95 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8>
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10>
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit TCC C5b-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
85 |
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| Range % |
80-92 |
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| 1:2 |
Average % |
96 |
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| Range % |
91-105 |
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| 1:4 |
Average % |
97 |
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| Range % |
92-103 |
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| 1:8 |
Average % |
91 |
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| Range % |
86-98 |
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| Recovery |
| The recovery of rabbit TCC C5b-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
97 |
89-109 |
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| EDTA plasma (n=4) |
97 |
92-108 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 500 |
2.659 |
2.563 |
2.611 |
2.519 |
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| 250 |
2.240 |
2.128 |
2.184 |
2.092 |
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| 125 |
1.488 |
1.375 |
1.432 |
1.340 |
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| 62.5 |
0.845 |
0.817 |
0.831 |
0.739 |
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| 31.2 |
0.444 |
0.434 |
0.439 |
0.347 |
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| 15.6 |
0.247 |
0.239 |
0.243 |
0.151 |
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| 7.8 |
0.156 |
0.148 |
0.152 |
0.060 |
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| 0 |
0.093 |
0.091 |
0.092 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
