Rabbit tissue inhibitors of metalloproteinase 1,TIMP-1 ELISA Kit
Product Details
| Target Name |
TIMP metallopeptidase inhibitor 1 |
| Alternative Names |
TIMP1 ELISA Kit; Metalloproteinase inhibitor 1 ELISA Kit; Tissue inhibitor of metalloproteinases 1 ELISA Kit; TIMP-1 ELISA Kit |
| Abbreviation |
TIMP1 |
| Species |
Oryctolagus cuniculus (Rabbit) |
| Sample Types |
serum, plasma, cell culture supernates , tissue homogenates |
| Detection Range |
15.6 pg/mL-1000 pg/mL |
| Sensitivity |
7.8 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Competitive |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit TIMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:100 |
Average % |
90 |
|
| Range % |
85-97 |
|
| 1:200 |
Average % |
102 |
|
| Range % |
98-108 |
|
| 1:400 |
Average % |
98 |
|
| Range % |
94-102 |
|
| 1:800 |
Average % |
88 |
|
| Range % |
84-93 |
|
|
| Recovery |
| The recovery of rabbit TIMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
95 |
89-99 |
|
| EDTA plasma (n=4) |
92 |
88-96 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
|
|
| 1000 |
0.086 |
0.084 |
0.085 |
|
|
| 500 |
0.145 |
0.141 |
0.143 |
|
|
| 250 |
0.245 |
0.238 |
0.242 |
|
|
| 125 |
0.392 |
0.413 |
0.403 |
|
|
| 62.5 |
0.725 |
0.727 |
0.726 |
|
|
| 31.2 |
0.963 |
0.944 |
0.954 |
|
|
| 15.6 |
1.154 |
1.179 |
1.167 |
|
|
| 0 |
1.734 |
1.662 |
1.698 |
|
|
|
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
