Rat Angiopoietin 1,ANG-1 ELISA Kit
Product Details
| Target Name |
angiopoietin 1 |
| Alternative Names |
Angpt1Angiopoietin-1 ELISA kit; ANG-1 ELISA kit |
| Abbreviation |
ANGPT1 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
6.25 ng/mL-400 ng/mL |
| Sensitivity |
1.56 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat ANG-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
104 |
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| Range % |
93-109 |
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| 1:2 |
Average % |
112 |
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| Range % |
103-116 |
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| 1:4 |
Average % |
94 |
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| Range % |
84-98 |
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| 1:8 |
Average % |
90 |
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| Range % |
83-94 |
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| Recovery |
| The recovery of rat ANG-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
90 |
83-94 |
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| EDTA plasma (n=4) |
103 |
92-108 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 400 |
2.985 |
2.845 |
2.915 |
2.781 |
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| 200 |
2.681 |
2.579 |
2.630 |
2.496 |
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| 100 |
2.297 |
2.213 |
2.255 |
2.121 |
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| 50 |
1.684 |
1.622 |
1.653 |
1.519 |
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| 25 |
1.001 |
0.956 |
0.979 |
0.845 |
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| 12.5 |
0.449 |
0.441 |
0.445 |
0.311 |
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| 6.25 |
0.251 |
0.242 |
0.247 |
0.113 |
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| 0 |
0.132 |
0.136 |
0.134 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
