Rat apolipoprotein A1 (Apo-A1) ELISA Kit
Product Details
| Target Name |
apolipoprotein A-I |
| Alternative Names |
Apoa1Apolipoprotein A-I ELISA kit; Apo-AI ELISA kit; ApoA-I ELISA kit; Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I ELISA kit; ProapoA-I)] ELISA kit |
| Abbreviation |
APOA1 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, bronchoalveolar lavage fluid, tissue homogenates |
| Detection Range |
0.156 μg/mL-10 μg/mL |
| Sensitivity |
0.039 μg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Apo-A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
|
| 1:200 |
Average % |
94 |
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| Range % |
87-99 |
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| 1:400 |
Average % |
92 |
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| Range % |
84-96 |
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| 1:800 |
Average % |
84 |
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| Range % |
85-106 |
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| 1:1600 |
Average % |
97 |
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| Range % |
91-105 |
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| Recovery |
| The recovery of rat Apo-A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
96 |
90-101 |
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| EDTA plasma (n=4) |
91 |
87-96 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| μg/m |
OD1 |
OD2 |
Average |
Corrected |
|
| 10 |
2.687 |
2.879 |
2.783 |
2.636 |
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| 5 |
2.481 |
2.490 |
2.486 |
2.339 |
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| 2.5 |
1.927 |
1.939 |
1.933 |
1.786 |
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| 1.25 |
1.353 |
1.387 |
1.370 |
1.223 |
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| 0.625 |
0.895 |
0.874 |
0.885 |
0.738 |
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| 0.312 |
0.592 |
0.571 |
0.582 |
0.435 |
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| 0.156 |
0.372 |
0.399 |
0.386 |
0.239 |
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| 0 |
0.148 |
0.146 |
0.147 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
