Rat Apolipoprotein A5(apo-A5)ELISA Kit
Product Details
| Target Name |
apolipoprotein A-V |
| Alternative Names |
Apoa5 ELISA kit; Rap3Apolipoprotein A-V ELISA kit; Apo-AV ELISA kit; ApoA-V ELISA kit; Apolipoprotein A5 ELISA kit; Regeneration-associated protein 3 ELISA kit |
| Abbreviation |
APOA5 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates_x000D_ |
| Detection Range |
31.2 pg/mL-2000 pg/mL |
| Sensitivity |
7.8 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Assay Principle |
quantitative |
| Measurement |
Competitive |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat apo-A5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
87 |
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| Range % |
84-90 |
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| 1:200 |
Average % |
99 |
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| Range % |
94-104 |
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| 1:400 |
Average % |
87 |
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| Range % |
84-90 |
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| 1:800 |
Average % |
100 |
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| Range % |
95-105 |
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| Recovery |
| The recovery of rat apo-A5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
93 |
90-95 |
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| EDTA plasma (n=4) |
94 |
90-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
1.945 |
1.987 |
1.966 |
1.871 |
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| 10 |
1.503 |
1.568 |
1.536 |
1.441 |
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| 5 |
1.115 |
1.042 |
1.079 |
0.984 |
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| 2.5 |
0.717 |
0.737 |
0.727 |
0.632 |
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| 1.25 |
0.406 |
0.415 |
0.411 |
0.316 |
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| 0.625 |
0.268 |
0.271 |
0.270 |
0.175 |
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| 0.312 |
0.161 |
0.163 |
0.162 |
0.067 |
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| 0 |
0.096 |
0.094 |
0.095 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
