Rat Aspartate Aminotransferase (AST) ELISA kit
Product Details
| Target Name |
glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) |
| Alternative Names |
Got1Aspartate aminotransferase ELISA kit; cytoplasmic ELISA kit; cAspAT ELISA kit; EC 2.6.1.1 ELISA kit; EC 2.6.1.3 ELISA kit; Cysteine aminotransferase ELISA kit; cytoplasmic ELISA kit; Cysteine transaminase ELISA kit; cytoplasmic ELISA kit; cCAT ELISA kit; Glutamate oxaloacetate transaminase 1 ELISA kit; Transaminase A ELISA kit |
| Abbreviation |
GOT1 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma |
| Detection Range |
3.12 mIU/mL-200 mIU/mL |
| Sensitivity |
0.78 mIU/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat AST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
87-92 |
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| 1:2 |
Average % |
104 |
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| Range % |
100-107 |
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| 1:4 |
Average % |
93 |
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| Range % |
88-97 |
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| 1:8 |
Average % |
98 |
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| Range % |
92-103 |
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| Recovery |
| The recovery of rat AST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
90-98 |
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| EDTA plasma (n=4) |
95 |
91-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| mIU/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 200 |
2.094 |
2.068 |
2.081 |
1.918 |
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| 100 |
1.276 |
1.310 |
1.293 |
1.130 |
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| 50 |
0.838 |
0.784 |
0.811 |
0.648 |
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| 25 |
0.521 |
0.539 |
0.530 |
0.367 |
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| 12.5 |
0.353 |
0.334 |
0.344 |
0.181 |
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| 6.25 |
0.300 |
0.288 |
0.294 |
0.131 |
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| 3.12 |
0.241 |
0.237 |
0.239 |
0.076 |
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| 0 |
0.164 |
0.162 |
0.163 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
