Rat Bone morphogenetic protein 2,BMP-2 ELISA Kit
Product Details
| Target Name |
bone morphogenetic protein 2 |
| Alternative Names |
Bmp2 ELISA kit; Bmp-2Bone morphogenetic protein 2 ELISA kit; BMP-2 ELISA kit; Bone morphogenetic protein 2A ELISA kit; BMP-2A ELISA kit |
| Abbreviation |
BMP2 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
7.8 pg/mL-500 pg/mL |
| Sensitivity |
1.95 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat BMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
95-110 |
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| 1:2 |
Average % |
84 |
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| Range % |
80-88 |
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| 1:4 |
Average % |
100 |
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| Range % |
95-106 |
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| 1:8 |
Average % |
98 |
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| Range % |
93-104 |
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| Recovery |
| The recovery of rat BMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
89 |
84-93 |
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| EDTA plasma (n=4) |
93 |
88-99 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 500 |
2.714 |
2.596 |
2.655 |
2.534 |
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| 250 |
2.212 |
2.142 |
2.177 |
2.056 |
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| 125 |
1.455 |
1.524 |
1.490 |
1.369 |
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| 62.5 |
0.800 |
0.789 |
0.795 |
0.674 |
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| 31.2 |
0.448 |
0.421 |
0.435 |
0.314 |
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| 15.6 |
0.253 |
0.243 |
0.248 |
0.127 |
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| 7.8 |
0.172 |
0.185 |
0.179 |
0.058 |
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| 0 |
0.123 |
0.118 |
0.121 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
