Rat Cardiac Troponin T(cTn-T)ELISA kit
Product Details
| Target Name |
Cardiac Troponin T(cTn-T) |
| Alternative Names |
Tnnt2 ELISA Kit; Troponin T ELISA Kit; cardiac muscle ELISA Kit; TnTc ELISA Kit; Cardiac muscle troponin T ELISA Kit; cTnT ELISA Kit |
| Abbreviation |
cTn-T |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
12.5 pg/mL-800 pg/mL |
| Sensitivity |
3.12 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat cTn-T in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
89 |
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| Range % |
82-97 |
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| 1:2 |
Average % |
87 |
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| Range % |
81-94 |
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| 1:4 |
Average % |
93 |
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| Range % |
87-100 |
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| 1:8 |
Average % |
95 |
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| Range % |
89-102 |
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| Recovery |
| The recovery of rat cTn-T spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
97 |
90-105 |
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| EDTA plasma (n=4) |
95 |
88-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 800 |
2.187 |
2.102 |
2.145 |
1.986 |
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| 400 |
1.731 |
1.641 |
1.686 |
1.527 |
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| 200 |
1.254 |
1.204 |
1.229 |
1.070 |
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| 100 |
0.926 |
0.879 |
0.903 |
0.744 |
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| 50 |
0.593 |
0.554 |
0.574 |
0.415 |
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| 25 |
0.302 |
0.279 |
0.291 |
0.132 |
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| 12.5 |
0.241 |
0.227 |
0.234 |
0.075 |
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| 0 |
0.165 |
0.153 |
0.159 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
