Product Details

Description

The product CSB-E13833r is a ready-to-use microwell, strip plate ELISA Kit for quantitative analysis of rat cartilage oligomeric matrix protein (COMP) in biological samples, including serum, plasma, or tissue homogenates. This kit has undergone rigorous quality tests in multiple parameters, including specificity, sensitivity, precision, and lot-to-lot consistency. The detection mechanism of this kit is based on the Sandwich-ELISA technique. COMP in the samples or standards are sandwiched between pre-coated COMP antibody and Biotin-conjugated COMP antibody. The COMP/pre-coated COMP antibody/Biotin-conjugated COMP antibody complex is labeled by HRP and then occurs a chromogenic reaction after the addition of the TMB solution. The color intensity is positively related to the COMP in the samples. COMP, also known as thrombospondin-5 (TSP-5), is a matricellular protein essential for maintaining vascular homeostasis. It also plays a crucial role in cartilage and bone metabolism and is involv...Read more

Target Name

cartilage oligomeric matrix protein

Alternative Names

CompCartilage oligomeric matrix protein ELISA kit; COMP ELISA kit

Abbreviation

COMP

Uniprot No.

P35444

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

78 pg/ml - 5000 pg/ml

Sensitivity

19.5 pg/ml

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cell Biology

Materials provided

A micro ELISA plate --The 96-well plate has been pre-coated with an anti-rat COMP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.

Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

One vial Biotin-labeled COMP antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.

One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.

One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.

One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.

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Materials not provided

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

An incubator can provide stable incubation conditions up to 37°C±5°C.

Centrifuge

Vortex

Squirt bottle, manifold dispenser, or automated microplate washer

Absorbent paper for blotting the microtiter plate

Storage

Store at 2-8°C. Please refer to protocol.