| Description |
The rat caspase 3 (CASP3) ELISA kit is suitable for quantitative measurement of the level of CASP3 protein in rat samples, including serum, plasma, and tissue homogenates. It provides a detection range of 0.312 ng/mL-20 ng/mL. It has a high sensitivity, as low as 0.078 ng/mL. Besides, this kit also possesses high precision, good linearity, excellent specificity, and high recovery. This assay is based on the sandwich enzyme immunoassay technique. Standards and samples are respectively added to the wells of the micro ELISA plate pre-coated with the CASP3 antibody. A biotin-conjugated CASP3 antibody is added to the wells, forming the sandwich-like complex CASP3 antibody/CASP3/biotin-conjugated CASP3. HRP-avidin is then added into the wells. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to CASP3 content bound in the initial step. The color development is stopped upon adding the stop solution, and the intens... Read more |
| Target Name |
caspase 3, apoptosis-related cysteine peptidase |
| Alternative Names |
Casp3 ELISA Kit; Cpp32Caspase-3 ELISA Kit; CASP-3 ELISA Kit; EC 3.4.22.56 ELISA Kit; Apopain ELISA Kit; Cysteine protease CPP32 ELISA Kit; CPP-32 ELISA Kit; IRP ELISA Kit; LICE ELISA Kit; Protein Yama ELISA Kit; SREBP cleavage activity 1 ELISA Kit; SCA-1) [Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12] ELISA Kit |
| Abbreviation |
CASP3 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.312 ng/mL-20 ng/mL |
| Sensitivity |
0.078 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cell Biology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Casp-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
84 |
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| Range % |
80-92 |
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| 1:2 |
Average % |
98 |
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| Range % |
91-105 |
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| 1:4 |
Average % |
99 |
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| Range % |
92-110 |
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| 1:8 |
Average % |
91 |
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| Range % |
86-98 |
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| Recovery |
| The recovery of rat Casp-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
95 |
89-98 |
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| EDTA plasma (n=4) |
96 |
90-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.915 |
2.903 |
2.909 |
2.828 |
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| 10 |
2.321 |
2.283 |
2.302 |
2.221 |
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| 5 |
1.467 |
1.444 |
1.456 |
1.375 |
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| 2.5 |
0.727 |
0.706 |
0.717 |
0.636 |
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| 1.25 |
0.365 |
0.359 |
0.362 |
0.281 |
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| 0.625 |
0.216 |
0.206 |
0.211 |
0.130 |
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| 0.312 |
0.137 |
0.129 |
0.133 |
0.052 |
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| 0 |
0.084 |
0.078 |
0.081 |
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| Materials provided |
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-Rat CASP3 antibody.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled CASP3 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
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| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
