rat Endothelin 1,ET-1 ELISA Kit
Product Details
| Target Name |
endothelin 1 |
| Alternative Names |
Edn1 ELISA Kit; Endothelin-1 ELISA Kit; ET-1 ELISA Kit; Preproendothelin-1 ELISA Kit; PPET1) [Cleaved into: Big endothelin-1] ELISA Kit |
| Abbreviation |
EDN1 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, cell lysates, tissue homogenates |
| Detection Range |
1.25 pg/mL-80 pg/mL |
| Sensitivity |
0.31 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat ET-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
85 |
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| Range % |
84-91 |
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| 1:2 |
Average % |
96 |
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| Range % |
91-102 |
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| 1:4 |
Average % |
84 |
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| Range % |
81-87 |
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| 1:8 |
Average % |
92 |
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| Range % |
88-96 |
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| Recovery |
| The recovery of rat ET-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
87-96 |
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| EDTA plasma (n=4) |
95 |
90-99 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 80 |
2.085 |
2.086 |
2.086 |
1.951 |
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| 40 |
1.540 |
1.649 |
1.595 |
1.460 |
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| 20 |
1.111 |
1.156 |
1.134 |
0.999 |
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| 10 |
0.718 |
0.750 |
0.734 |
0.599 |
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| 5 |
0.407 |
0.405 |
0.406 |
0.271 |
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| 2.5 |
0.231 |
0.237 |
0.234 |
0.099 |
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| 1.25 |
0.181 |
0.173 |
0.177 |
0.042 |
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| 0 |
0.129 |
0.140 |
0.135 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
