Rat Epithelial-Cadherin,E-Cad ELISA Kit
Product Details
| Target Name |
cadherin 1, type 1, E-cadherin (epithelial) |
| Alternative Names |
Cdh1Cadherin-1 ELISA kit; Epithelial cadherin ELISA kit; E-cadherin ELISA kit; Uvomorulin ELISA kit; CD antigen CD324) [Cleaved into: E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3] ELISA kit |
| Abbreviation |
CDH1 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
3.12 ng/mL-200 ng/mL |
| Sensitivity |
0.78 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat E-Cad in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
87 |
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| Range % |
85-90 |
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| 1:2 |
Average % |
102 |
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| Range % |
98-106 |
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| 1:4 |
Average % |
99 |
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| Range % |
95-103 |
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| 1:8 |
Average % |
83 |
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| Range % |
80-86 |
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| Recovery |
| The recovery of rat E-Cad spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
105 |
101-109 |
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| EDTA plasma (n=4) |
97 |
93-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 200 |
2.750 |
2.798 |
2.774 |
2.591 |
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| 100 |
2.045 |
1.968 |
2.007 |
1.824 |
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| 50 |
1.401 |
1.358 |
1.380 |
1.197 |
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| 25 |
0.920 |
0.967 |
0.944 |
0.761 |
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| 12.5 |
0.576 |
0.598 |
0.587 |
0.404 |
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| 6.25 |
0.445 |
0.468 |
0.457 |
0.274 |
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| 3.12 |
0.307 |
0.312 |
0.310 |
0.127 |
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| 0 |
0.182 |
0.183 |
0.183 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
