The Rat follicle-stimulating hormone (FSH) ELISA Kit is designed for the quantitative determination of endogenic rat FSH concentrations in serum, plasma, or tissue homogenates. This assay employs the competitive inhibition enzyme immunoassay technique. Standards or samples are added to the appropriate goat-anti-rat FSH antibody-pre-coated microtiter plate wells with the anti-rat FSH antibody and HRP-conjugate. The competitive inhibition reaction is launched between with HRP labeled FSH and FSH in the samples or standards with the anti-rat FSH antibody. These Ag-Ab complexes are captured by the antibody immobilized on the plate. Substrate A and B solution are mixed and then added to the wells, and the color turns blue. The color development is stopped after adding the stop solution, and the intensity of the color is opposite to the amount of FSH in the sample. Read the OD value with the Microplate reader and calculated the FSH concentration referring to the standard curve. This kit has been quality tested by multiple criteria, including sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency. Click the product instructions for more information.

FSH is a pituitary-produced glycoprotein gonadotropin that plays a critical role in mammalian reproduction and development. It is responsive to gonadotropin-releasing hormone (GnRH) released by the hypothalamus. In the ovary, FSH regulates folliculogenesis, oocyte selection, and the synthesis of sex steroid hormones, thus preparing the reproductive tract for fertilization, implantation, and pregnancy. FSH is required for testicular development and normal spermatogenesis in men.