Rat glucagon-like peptide-1,GLP-1 ELISA Kit
Product Details
| Target Name |
glucagon-like peptide-1,GLP-1 |
| Alternative Names |
N/A |
| Abbreviation |
Glp1 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
0.625 pg/mL-40 pg/mL |
| Sensitivity |
0.156 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Metabolism |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat GLP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
86 |
|
| Range % |
84-95 |
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| 1:200 |
Average % |
95 |
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| Range % |
91-100 |
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| 1:400 |
Average % |
84 |
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| Range % |
81-89 |
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| 1:800 |
Average % |
92 |
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| Range % |
86-98 |
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| Recovery |
| The recovery of rat GLP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
91 |
87-96 |
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| EDTA plasma (n=4) |
103 |
95-108 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 40 |
2.607 |
2.744 |
2.676 |
2.531 |
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| 20 |
2.303 |
2.451 |
2.377 |
2.232 |
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| 10 |
1.817 |
1.922 |
1.870 |
1.725 |
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| 5 |
1.133 |
1.191 |
1.162 |
1.017 |
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| 2.5 |
0.621 |
0.632 |
0.627 |
0.482 |
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| 1.25 |
0.344 |
0.355 |
0.350 |
0.205 |
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| 0.625 |
0.234 |
0.248 |
0.241 |
0.096 |
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| 0 |
0.140 |
0.150 |
0.145 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
