Rat glucose transporter 4(GLUT4) ELISA Kit
Product Details
| Target Name |
solute carrier family 2 (facilitated glucose transporter), member 4 |
| Alternative Names |
Slc2a4 ELISA Kit; Glut4Solute carrier family 2 ELISA Kit; facilitated glucose transporter member 4 ELISA Kit; Glucose transporter type 4 ELISA Kit; insulin-responsive ELISA Kit; GLUT-4 ELISA Kit |
| Abbreviation |
SLC2A4 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
0.156 ng/mL-10 ng/mL |
| Sensitivity |
0.039 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat GLUT4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
87 |
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| Range % |
80-92 |
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| 1:2 |
Average % |
98 |
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| Range % |
91-105 |
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| 1:4 |
Average % |
101 |
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| Range % |
92-110 |
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| 1:8 |
Average % |
93 |
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| Range % |
86-98 |
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| Recovery |
| The recovery of rat GLUT4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 10 |
2.958 |
3.000 |
2.979 |
2.850 |
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| 5 |
2.461 |
2.460 |
2.461 |
2.332 |
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| 2.5 |
1.852 |
1.876 |
1.864 |
1.735 |
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| 1.25 |
1.234 |
1.261 |
1.248 |
1.119 |
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| 0.625 |
0.753 |
0.798 |
0.776 |
0.647 |
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| 0.312 |
0.531 |
0.578 |
0.555 |
0.426 |
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| 0.156 |
0.396 |
0.412 |
0.404 |
0.275 |
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| 0 |
0.132 |
0.126 |
0.129 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
