Rat Glutathione Peroxidase (GSH-PX) ELISA kit
Product Details
| Target Name |
glutathione peroxidase 1 |
| Alternative Names |
Gpx1Glutathione peroxidase 1 ELISA Kit; GPx-1 ELISA Kit; GSHPx-1 ELISA Kit; EC 1.11.1.9 ELISA Kit; Cellular glutathione peroxidase ELISA Kit |
| Abbreviation |
GPX1 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
12.5 mIU/mL-800 mIU/mL |
| Sensitivity |
3.12 mIU/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat GSH-PX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:200 |
Average % |
92 |
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| Range % |
85-100 |
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| 1:400 |
Average % |
97 |
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| Range % |
94-101 |
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| 1:800 |
Average % |
95 |
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| Range % |
90-99 |
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| 1:1600 |
Average % |
93 |
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| Range % |
87-98 |
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| Recovery |
| The recovery of rat GSH-PX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
94 |
86-100 |
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| EDTA plasma (n=4) |
100 |
95-105 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| mIU/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 800 |
2.251 |
2.299 |
2.275 |
2.119 |
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| 400 |
1.845 |
1.834 |
1.840 |
1.684 |
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| 200 |
1.301 |
1.324 |
1.313 |
1.157 |
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| 100 |
0.892 |
0.876 |
0.884 |
0.728 |
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| 50 |
0.553 |
0.590 |
0.572 |
0.416 |
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| 25 |
0.395 |
0.381 |
0.388 |
0.232 |
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| 12.5 |
0.251 |
0.234 |
0.243 |
0.087 |
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| 0 |
0.155 |
0.157 |
0.156 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
