Rat Granulocyte Colony Stimulating Factor,G-CSF ELISA Kit
Product Details
| Target Name |
Granulocyte Colony Stimulating Factor,G-CSF |
| Alternative Names |
Csf2 ELISA kit; CsfgmGranulocyte-macrophage colony-stimulating factor ELISA kit; GM-CSF ELISA kit; Colony-stimulating factor ELISA kit; CSF ELISA kit |
| Abbreviation |
G-CSF |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
31.25 pg/mL-2000 pg/mL |
| Sensitivity |
7.81 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat G-CSF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
91 |
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| Range % |
86-94 |
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| 1:2 |
Average % |
101 |
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| Range % |
97-104 |
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| 1:4 |
Average % |
95 |
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| Range % |
90-97 |
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| 1:8 |
Average % |
100 |
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| Range % |
95-103 |
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| Recovery |
| The recovery of rat G-CSF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
94 |
90-96 |
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| EDTA plasma (n=4) |
94 |
92-96 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.133 |
2.118 |
2.126 |
2.007 |
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| 1000 |
1.837 |
1.776 |
1.807 |
1.688 |
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| 500 |
1.294 |
1.282 |
1.288 |
1.169 |
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| 250 |
0.889 |
0.878 |
0.884 |
0.765 |
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| 125 |
0.588 |
0.607 |
0.598 |
0.479 |
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| 62.5 |
0.361 |
0.349 |
0.355 |
0.236 |
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| 31.25 |
0.222 |
0.216 |
0.219 |
0.100 |
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| 0 |
0.118 |
0.120 |
0.119 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
