The Rat growth hormone (GH) ELISA KIT is used to quantitatively measure the levels of CEA in human serum, plasma, cell culture supernates, or tissue homogenates. This assay employs the quantitative sandwich enzyme immunoassay technique, in which GH in the samples or standards are sandwiched between pre-coated GH antibody and Biotin-conjugated antibody specific for GH. The Ab-Ag-Ab complex is labeled by HRP. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of GH bound in the initial step. The color development is stopped and the intensity of the color is measured at 450 nm via a microplate reader. This kit displays many advantages, including sensitivity, specificity, precision, linearity, and recovery. The product instructions are access to more information.

GH is a pituitary gland-produced peptide hormone that spurs growth, cell reproduction, body composition, cell repair, and metabolism in mammalians. The secretion of GH is a part of a negative feedback loop involving IGF-1. Its secretion is stimulated by the growth hormone-releasing hormone (GHRH) and is suppressed by somatostatin. High blood levels of IGF-1 cause a decrease in GH secretion not only directly inhibiting the somatotroph but by stimulating somatostatin release from the hypithalamus. GH deficiency, one of the many causes of dwarfism and short stature, is caused mainly by damage to the hypithalamus or the pituitary gland during fetal development or following birth.