Rat heart fatty acid binding protein(h-FABP)ELISA Kit
Product Details
| Target Name |
fatty acid binding protein 3, muscle and heart (mammary-derived growth inhibitor) |
| Alternative Names |
Fabp3Fatty acid-binding protein ELISA kit; heart ELISA kit; Fatty acid-binding protein 3 ELISA kit; Heart-type fatty acid-binding protein ELISA kit; H-FABP ELISA kit |
| Abbreviation |
FABP3 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
15.6 pg/mL-1000 pg/mL |
| Sensitivity |
3.9 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat h-FABP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
85 |
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| Range % |
84-92 |
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| 1:2 |
Average % |
96 |
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| Range % |
91-102 |
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| 1:4 |
Average % |
84 |
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| Range % |
81-88 |
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| 1:8 |
Average % |
94 |
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| Range % |
90-98 |
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| Recovery |
| The recovery of rat h-FABP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
92 |
87-95 |
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| EDTA plasma (n=4) |
103 |
95-108 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.095 |
2.086 |
2.091 |
1.967 |
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| 500 |
1.550 |
1.648 |
1.599 |
1.475 |
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| 250 |
1.121 |
1.156 |
1.139 |
1.015 |
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| 125 |
0.728 |
0.751 |
0.740 |
0.616 |
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| 62.5 |
0.417 |
0.403 |
0.410 |
0.286 |
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| 31.2 |
0.241 |
0.237 |
0.239 |
0.115 |
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| 15.6 |
0.211 |
0.214 |
0.213 |
0.089 |
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| 0 |
0.119 |
0.129 |
0.124 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
