Rat heat shock protein 70,hSP-70 ELISA Kit
Product Details
| Target Name |
heat shock 70kDa protein 1A |
| Abbreviation |
HSPA1A |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
62.5 pg/mL-4000 pg/mL |
| Sensitivity |
15.6 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Neuroscience |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8>
| |
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV%<10>
| |
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat hSP-70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:100 |
Average % |
92 |
|
| Range % |
88-95 |
|
| 1:200 |
Average % |
103 |
|
| Range % |
99-107 |
|
| 1:400 |
Average % |
92 |
|
| Range % |
86-96 |
|
| 1:800 |
Average % |
98 |
|
| Range % |
94-101 |
|
|
| Recovery |
| The recovery of rat hSP-70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
94 |
91-97 |
|
| EDTA plasma (n=4) |
95 |
91-98 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 4000 |
2.402 |
2.333 |
2.368 |
2.226 |
|
| 2000 |
1.946 |
1.926 |
1.936 |
1.794 |
|
| 1000 |
1.422 |
1.433 |
1.428 |
1.286 |
|
| 500 |
0.911 |
0.924 |
0.918 |
0.776 |
|
| 250 |
0.477 |
0.491 |
0.484 |
0.342 |
|
| 125 |
0.332 |
0.323 |
0.328 |
0.186 |
|
| 62.5 |
0.244 |
0.237 |
0.241 |
0.099 |
|
| 0 |
0.143 |
0.141 |
0.142 |
|
|
|
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
