Rat heme oxygenase 1,HO-1 ELISA Kit
Product Details
| Target Name |
heme oxygenase (decycling) 1 |
| Alternative Names |
Hmox1Heme oxygenase 1 ELISA Kit; HO-1 ELISA Kit; EC 1.14.14.18 ELISA Kit; HSP32 ELISA Kit |
| Abbreviation |
HMOX1 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.312 ng/mL-20 ng/mL |
| Sensitivity |
0.078 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat HO-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
98 |
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| Range % |
94-102 |
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| 1:2 |
Average % |
100 |
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| Range % |
96-107 |
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| 1:4 |
Average % |
86 |
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| Range % |
82-90 |
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| 1:8 |
Average % |
94 |
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| Range % |
90-100 |
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| Recovery |
| The recovery of rat HO-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
91 |
87-99 |
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| EDTA plasma (n=4) |
96 |
91-103 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.323 |
2.252 |
2.288 |
2.185 |
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| 10 |
1.776 |
1.692 |
1.734 |
1.631 |
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| 5 |
1.182 |
1.105 |
1.144 |
1.041 |
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| 2.5 |
0.725 |
0.743 |
0.734 |
0.631 |
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| 1.25 |
0.465 |
0.443 |
0.454 |
0.351 |
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| 0.625 |
0.275 |
0.259 |
0.267 |
0.164 |
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| 0.312 |
0.192 |
0.207 |
0.200 |
0.097 |
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| 0 |
0.104 |
0.102 |
0.103 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
