Product Details

Description

The Rat Immunoglobulin G (IgG) ELISA Kit is specifically designed to standardize the detection of rat IgG in serum and plasma. This kit employs the Competitive-ELISA mechanism to quantitatively measure the concentration of IgG. The rat IgG has been pre-coated on the microtiter plate. Standards and samples are pipetted into the wells with HRP-conjugated antibody specific for rat IgG. A competitive inhibition reaction is launched between IgG (standards or samples) and pre-coated IgG with HRP-conjugated IgG antibody. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells. HRP-TMB interaction elicits a chromogenic reaction. The color development is terminated, and the solution color changes from blue to yellow. The color intensity is in opposition to the amount of rat IgG in samples. This kit has been professionally verified with reliable quality, high sensitivity, excellent specificity, good linearity, precision less than 10%, and consistency betw...Read more

Target Name

Immunoglobulin G,IgG

Alternative Names

N/A

Abbreviation

IgG

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma

Detection Range

31.25 ng/mL-8000 ng/mL

Sensitivity

31.25 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Competitive

Precision

• Intra-assay Precision (Precision within an assay): CV%
• Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision (Precision between assays): CV%
• Three samples of known concentration were tested in twenty assays to assess.