Rat inhibin B,INH-B ELISA Kit
Product Details
| Target Name |
inhibin B,INH-B |
| Alternative Names |
N/A |
| Abbreviation |
INH-B |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
1 pg/mL-200 pg/mL |
| Sensitivity |
0.5 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Competitive |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat INH-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
95 |
|
| Range % |
90-106 |
|
| 1:2 |
Average % |
98 |
|
| Range % |
90-105 |
|
| 1:4 |
Average % |
94 |
|
| Range % |
89-99 |
|
| 1:8 |
Average % |
96 |
|
| Range % |
90-100 |
|
|
| Recovery |
| The recovery of rat INH-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
96 |
87-104 |
|
| EDTA plasma (n=4) |
97 |
91-106 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
|
|
| 200 |
0.240 |
0.246 |
0.243 |
|
|
| 50 |
0.291 |
0.305 |
0.298 |
|
|
| 16 |
0.421 |
0.432 |
0.427 |
|
|
| 4 |
0.722 |
0.697 |
0.710 |
|
|
| 1 |
1.117 |
1.146 |
1.132 |
|
|
| 0 |
2.188 |
2.164 |
2.176 |
|
|
|
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
