Rat Interleukin 10,IL-10 ELISA KIT
Product Details
| Target Name |
interleukin 10 |
| Alternative Names |
Il10 ELISA Kit; Il-10Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit |
| Abbreviation |
IL10 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
3.12 pg/mL-200 pg/mL |
| Sensitivity |
0.78 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
88-98 |
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| 1:2 |
Average % |
96 |
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| Range % |
91-101 |
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| 1:4 |
Average % |
90 |
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| Range % |
85-95 |
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| 1:8 |
Average % |
93 |
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| Range % |
90-96 |
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| Recovery |
| The recovery of rat IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
87-98 |
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| EDTA plasma (n=4) |
95 |
90-102 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 200 |
2.611 |
2.671 |
2.641 |
2.474 |
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| 100 |
1.873 |
1.867 |
1.870 |
1.703 |
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| 50 |
1.206 |
1.234 |
1.220 |
1.053 |
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| 25 |
0.804 |
0.817 |
0.811 |
0.644 |
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| 12.5 |
0.515 |
0.527 |
0.521 |
0.354 |
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| 6.25 |
0.387 |
0.399 |
0.393 |
0.226 |
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| 3.12 |
0.314 |
0.327 |
0.321 |
0.154 |
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| 0 |
0.155 |
0.179 |
0.167 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
