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Rat Interleukin 13,IL-13 ELISA KIT

Product Details

Description

The product CSB-E07454r is a ready-to-use microwell, strip plate ELISA Kit for quantitative analysis of rat interleukin 13(IL-13)in biological samples, including serum, plasma, cell culture supernates, and tissue homogenates. Based on the Sandwich-ELISA technique in combination with the enzyme-substrate chromogenic reaction, the color intensity is proportional tothe amount of IL-13 bound in the initial step. IL-13 levels can be calculated according to the standard curve.

This rat IL-13 ELISA Kit has been verified by multiple tests with high sensitivity, strong specificity, precision less than 10%, high recovery, and lot-to-lot consistency.

IL-13 is a pleiotropic cytokine produced by activated Th2 cells, lymphoid cells (ILC2s), mast cells, eosinophils, basophils, macrophages, and B cells. It acts through the IL-13Ra1/IL-4Ra complex to induce activation responses which contribute to the inflammatory diseases. IL-13 and the closely related cytokine IL-4 shares many immunoregulatory functions, including regulation of antibody production and inflammation. It acts as a central mediator in allergy and antihelminth defense with various effects. Besides, IL-13 also mediates goblet cell hyperplasia, airway smooth muscle contractility, collagen deposition, and fibrosis.
Target Name interleukin 13
Alternative Names Il13 ELISA Kit; Il-13 ELISA Kit; Interleukin-13 ELISA Kit; IL-13 ELISA Kit; T-cell activation protein P600 ELISA Kit
Abbreviation IL13
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 7.8 pg/mL-500 pg/mL
Sensitivity 1.95 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%   
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%   
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)  
1:1 Average % 91  
Range % 88-93  
1:2 Average % 104  
Range % 99-107  
1:4 Average % 93  
Range % 88-97  
1:8 Average % 99  
Range % 91-103  
Recovery
The recovery of rat IL-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-98  
EDTA plasma (n=4) 94 91-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
500 1.854 1.766 1.810 1.727  
250 1.117 1.106 1.112 1.029  
125 0.728 0.743 0.736 0.653  
62.5 0.517 0.534 0.526 0.443  
31.2 0.303 0.312 0.308 0.225  
15.6 0.210 0.202 0.206 0.123  
7.8 0.171 0.164 0.168 0.085  
0 0.084 0.082 0.083 　  
Materials provided
  • A micro ELISA plate --The 96-well plate has been pre-coated with an anti-rat IL-13 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labeled IL-13 antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
  • One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
  • One vial Sample Diluent (50 ml/bottle)--Dilute the sample to an appropriate concentration.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) --Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
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