Rat Interleukin 15,IL-15 ELISA KIT
Product Details
| Target Name |
interleukin 15 |
| Alternative Names |
Il15 ELISA Kit; Interleukin-15 ELISA Kit; IL-15 ELISA Kit |
| Abbreviation |
IL15 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
1.56 pg/mL-100 pg/mL |
| Sensitivity |
0.39 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
96 |
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| Range % |
90-101 |
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| 1:2 |
Average % |
93 |
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| Range % |
87-99 |
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| 1:4 |
Average % |
87 |
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| Range % |
82-94 |
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| 1:8 |
Average % |
100 |
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| Range % |
95-104 |
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| Recovery |
| The recovery of rat IL-15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
96 |
91-101 |
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| EDTA plasma (n=4) |
93 |
87-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 100 |
2.511 |
2.567 |
2.539 |
2.383 |
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| 50 |
1.773 |
1.783 |
1.778 |
1.622 |
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| 25 |
1.106 |
1.154 |
1.130 |
0.974 |
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| 12.5 |
0.704 |
0.725 |
0.715 |
0.559 |
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| 6.25 |
0.415 |
0.431 |
0.423 |
0.267 |
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| 3.12 |
0.287 |
0.297 |
0.292 |
0.136 |
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| 1.56 |
0.214 |
0.231 |
0.223 |
0.067 |
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| 0 |
0.155 |
0.157 |
0.156 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
