Rat Interleukin 16,IL-16 ELISA KIT
Product Details
| Target Name |
Interleukin 16,IL-16 |
| Abbreviation |
IL16 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
31.25 pg/mL-2000 pg/mL |
| Sensitivity |
7.81 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-16 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
87-96 |
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| 1:2 |
Average % |
98 |
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| Range % |
94-102 |
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| 1:4 |
Average % |
103 |
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| Range % |
98-107 |
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| 1:8 |
Average % |
89 |
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| Range % |
84-96 |
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| Recovery |
| The recovery of rat IL-16 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
90-98 |
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| EDTA plasma (n=4) |
88 |
82-96 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.757 |
2.656 |
2.707 |
2.524 |
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| 1000 |
2.223 |
2.122 |
2.173 |
1.990 |
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| 500 |
1.488 |
1.467 |
1.478 |
1.295 |
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| 250 |
0.937 |
0.926 |
0.932 |
0.749 |
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| 125 |
0.589 |
0.578 |
0.584 |
0.401 |
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| 62.5 |
0.425 |
0.414 |
0.420 |
0.237 |
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| 31.25 |
0.305 |
0.297 |
0.301 |
0.118 |
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| 0 |
0.183 |
0.182 |
0.183 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
