Rat Interleukin 17,IL-17 ELISA KIT
Product Details
| Target Name |
interleukin 17A |
| Alternative Names |
Il17a ELISA Kit; Ctla8 ELISA Kit; Il17Interleukin-17A ELISA Kit; IL-17 ELISA Kit; IL-17A ELISA Kit; Cytotoxic T-lymphocyte-associated antigen 8 ELISA Kit; CTLA-8 ELISA Kit |
| Abbreviation |
IL17A |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
93.75 pg/mL-6000 pg/mL |
| Sensitivity |
23.43 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
87 |
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| Range % |
80-95 |
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| 1:2 |
Average % |
85 |
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| Range % |
80-95 |
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| 1:4 |
Average % |
90 |
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| Range % |
86-100 |
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| 1:8 |
Average % |
92 |
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| Range % |
86-97 |
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| Recovery |
| The recovery of rat IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
90 |
85-98 |
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| EDTA plasma (n=4) |
93 |
88-101 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 6000 |
1.865 |
1.802 |
1.834 |
1.739 |
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| 3000 |
1.418 |
1.376 |
1.397 |
1.302 |
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| 1500 |
1.036 |
1.004 |
1.020 |
0.925 |
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| 750 |
0.624 |
0.589 |
0.607 |
0.512 |
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| 375 |
0.379 |
0.364 |
0.372 |
0.277 |
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| 187.5 |
0.211 |
0.209 |
0.210 |
0.115 |
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| 93.75 |
0.132 |
0.122 |
0.127 |
0.032 |
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| 0 |
0.099 |
0.091 |
0.095 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
