Rat Interleukin 18,IL-18 Elisa Kit
Product Details
| Target Name |
interleukin 18 (interferon-gamma-inducing factor) |
| Alternative Names |
Il18 ELISA Kit; Igif ELISA Kit; Interleukin-18 ELISA Kit; IL-18 ELISA Kit; Interferon gamma-inducing factor ELISA Kit; IFN-gamma-inducing factor ELISA Kit; Interleukin-1 gamma ELISA Kit; IL-1 gamma ELISA Kit |
| Abbreviation |
IL18 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, cell culture supernates |
| Detection Range |
0.156 ng/mL-10 ng/mL |
| Sensitivity |
0.039 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
96 |
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| Range % |
90-102 |
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| 1:2 |
Average % |
89 |
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| Range % |
84-95 |
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| 1:4 |
Average % |
100 |
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| Range % |
95-106 |
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| 1:8 |
Average % |
97 |
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| Range % |
92-104 |
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| Recovery |
| The recovery of rat IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
89 |
93-96 |
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| EDTA plasma (n=4) |
102 |
98-107 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 10 |
2.357 |
2.387 |
2.372 |
2.218 |
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| 5 |
1.686 |
1.699 |
1.693 |
1.539 |
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| 2.5 |
1.064 |
1.015 |
1.040 |
0.886 |
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| 1.25 |
0.687 |
0.654 |
0.671 |
0.517 |
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| 0.625 |
0.382 |
0.398 |
0.390 |
0.236 |
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| 0.312 |
0.289 |
0.270 |
0.280 |
0.126 |
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| 0.156 |
0.221 |
0.235 |
0.228 |
0.074 |
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| 0 |
0.151 |
0.157 |
0.154 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
