Rat Interleukin 21,IL-21 ELISA Kit
Product Details
| Target Name |
interleukin 21 |
| Alternative Names |
Il21Interleukin-21 ELISA Kit; IL-21 ELISA Kit |
| Abbreviation |
IL21 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
15.6 pg/mL-1000 pg/mL |
| Sensitivity |
3.9 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-21 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
91 |
|
| Range % |
85-96 |
|
| 1:2 |
Average % |
89 |
|
| Range % |
84-95 |
|
| 1:4 |
Average % |
103 |
|
| Range % |
99-108 |
|
| 1:8 |
Average % |
99 |
|
| Range % |
94-105 |
|
|
| Recovery |
| The recovery of rat IL-21 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
97 |
92-101 |
|
| EDTA plasma (n=4) |
92 |
88-100 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 1000 |
2.601 |
2.544 |
2.573 |
2.456 |
|
| 500 |
1.921 |
1.952 |
1.937 |
1.820 |
|
| 250 |
1.198 |
1.122 |
1.160 |
1.043 |
|
| 125 |
0.735 |
0.709 |
0.722 |
0.605 |
|
| 62.5 |
0.425 |
0.459 |
0.442 |
0.325 |
|
| 31.2 |
0.272 |
0.257 |
0.265 |
0.148 |
|
| 15.6 |
0.181 |
0.172 |
0.177 |
0.060 |
|
| 0 |
0.116 |
0.118 |
0.117 |
|
|
|
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
