Rat Interleukin 22(IL-22) ELISA Kit
Product Details
| Target Name |
Interleukin 22(IL-22) |
| Abbreviation |
IL-22 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
15.6 pg/mL-1000 pg/mL |
| Sensitivity |
3.9 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-22 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
88 |
|
| Range % |
83-94 |
|
| 1:2 |
Average % |
99 |
|
| Range % |
90-102 |
|
| 1:4 |
Average % |
93 |
|
| Range % |
85-97 |
|
| 1:8 |
Average % |
92 |
|
| Range % |
84-96 |
|
|
| Recovery |
| The recovery of rat IL-22 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
101 |
95-105 |
|
| EDTA plasma (n=4) |
104 |
92-109 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 1000 |
2.120 |
2.147 |
2.134 |
1.958 |
|
| 500 |
1.493 |
1.486 |
1.490 |
1.314 |
|
| 250 |
1.029 |
1.105 |
1.067 |
0.891 |
|
| 125 |
0.658 |
0.673 |
0.666 |
0.490 |
|
| 62.5 |
0.456 |
0.444 |
0.450 |
0.274 |
|
| 31.2 |
0.346 |
0.358 |
0.352 |
0.176 |
|
| 15.6 |
0.270 |
0.262 |
0.266 |
0.090 |
|
| 0 |
0.174 |
0.178 |
0.176 |
|
|
|
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
