Rat Interleukin 23,IL-23 ELISA Kit
Product Details
| Target Name |
Interleukin 23,IL-23 |
| Alternative Names |
N/A |
| Abbreviation |
IL-23 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
3.12 pg/mL-200 pg/mL |
| Sensitivity |
0.78 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
89-94 |
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| 1:2 |
Average % |
104 |
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| Range % |
100-107 |
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| 1:4 |
Average % |
92 |
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| Range % |
87-95 |
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| Recovery |
| The recovery of rat IL-23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
89-95 |
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| EDTA plasma (n=4) |
98 |
94-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 200 |
2.147 |
2.112 |
2.130 |
1.998 |
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| 100 |
1.851 |
1.776 |
1.814 |
1.682 |
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| 50 |
1.308 |
1.288 |
1.298 |
1.166 |
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| 25 |
0.903 |
0.898 |
0.901 |
0.769 |
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| 12.5 |
0.621 |
0.614 |
0.618 |
0.486 |
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| 6.25 |
0.375 |
0.359 |
0.367 |
0.235 |
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| 3.12 |
0.236 |
0.224 |
0.230 |
0.098 |
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| 0 |
0.134 |
0.130 |
0.132 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
