Rat Interleukin 2,IL-2 ELISA kit
Product Details
| Target Name |
interleukin 2 |
| Alternative Names |
Il2 ELISA kit; Il-2Interleukin-2 ELISA kit; IL-2 ELISA kit; T-cell growth factor ELISA kit; TCGF ELISA kit |
| Abbreviation |
IL2 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, urine, tissue homogenates |
| Detection Range |
6.25 pg/mL-400 pg/mL |
| Sensitivity |
1.56 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
87-98 |
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| 1:2 |
Average % |
98 |
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| Range % |
91-104 |
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| 1:4 |
Average % |
89 |
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| Range % |
84-99 |
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| 1:8 |
Average % |
96 |
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| Range % |
92-100 |
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| Recovery |
| The recovery of rat IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
90 |
85-96 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 400 |
2.489 |
2.478 |
2.484 |
2.289 |
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| 200 |
1.954 |
1.896 |
1.925 |
1.730 |
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| 100 |
1.292 |
1.273 |
1.283 |
1.088 |
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| 50 |
0.797 |
0.768 |
0.783 |
0.588 |
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| 25 |
0.503 |
0.489 |
0.496 |
0.301 |
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| 12.5 |
0.364 |
0.356 |
0.360 |
0.165 |
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| 6.25 |
0.276 |
0.258 |
0.267 |
0.072 |
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| 0 |
0.198 |
0.192 |
0.195 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
