Rat Interleukin 33(IL-33) ELISA Kit
Product Details
| Target Name |
interleukin 33 |
| Alternative Names |
Il33Interleukin-33 ELISA kit; IL-33 ELISA kit |
| Abbreviation |
IL33 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
15.6 pg/mL-1000 pg/mL |
| Sensitivity |
3.9 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-33 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
103 |
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| Range % |
98-108 |
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| 1:2 |
Average % |
96 |
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| Range % |
91-101 |
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| 1:4 |
Average % |
89 |
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| Range % |
83-95 |
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| 1:8 |
Average % |
98 |
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| Range % |
93-104 |
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| Recovery |
| The recovery of rat IL-33 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
89 |
85-94 |
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| EDTA plasma (n=4) |
94 |
90-99 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.981 |
2.998 |
2.990 |
2.842 |
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| 500 |
1.967 |
1.873 |
1.920 |
1.772 |
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| 250 |
1.333 |
1.427 |
1.380 |
1.232 |
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| 125 |
0.845 |
0.835 |
0.840 |
0.692 |
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| 62.5 |
0.565 |
0.559 |
0.562 |
0.414 |
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| 31.2 |
0.331 |
0.348 |
0.340 |
0.192 |
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| 15.6 |
0.225 |
0.236 |
0.231 |
0.083 |
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| 0 |
0.143 |
0.153 |
0.148 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
