Rat Interleukin 6,IL-6 ELISA KIT
Product Details
| Description |
This Rat Interleukin 6 (IL-6) ELISA Kit is suitable for qualitatively determining IL-6 concentrations in muliple biological fluids, including rat serum, plasma, and tissue homogenates in vitro. IL-6 is a pleiotropic pro-inflammatory cytokine, and its production is responsive to tissue injury and infections. It is involved in diverse biological processes such as the modulation of the host immune response, the progression of several virus infectons, and the regulation of immune cell proliferation and differentiation. IL-6 exerts its biological roles through a hexameric complex IL-6/IL-6R/gp130, which activates different signaling pathways such as JAK/STAT3, Ras/MAPK, PI3K–PKB/Akt to execute various biochemical functions. Deregulation of IL-6 signaling is associated with chronic inflammation, and multifactorial auto-immune disorders. This kit uses the quantitative sandwich-based enzyme immunoassay technique to measure the amount of rat IL-6 in the sample. Standards and samples are respect... Read more |
| Alternative Names |
Il6 ELISA Kit; Il-6 ELISA Kit; Interleukin-6 ELISA Kit; IL-6 ELISA Kit |
| Abbreviation |
IL6 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.312 pg/mL-20 pg/mL |
| Sensitivity |
0.078 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
| Inter-assay Precision (Precision between assays): CV%0% |
| Three samples of known concentration were tested in twenty assays to assess. |
|
| Linearity |
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
| |
Sample |
Serum(n=4) |
| 1:2 |
Average % |
86 |
| |
Range % |
84-95 |
| 1:4 |
Average % |
95 |
| |
Range % |
91-100 |
| 1:8 |
Average % |
84 |
| |
Range % |
81-89 |
| 1:16 |
Average % |
92 |
| |
Range % |
87-96 |
|
| Recovery |
The recovery of rat IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
| Sample Type |
Average % Recovery |
Range |
| Serum (n=5) |
95 |
89-100 |
| EDTA plasma (n=4) |
96 |
92-99 |
|
| Typical Data |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
| 20 |
2.524 |
2.615 |
2.570 |
2.420 |
| 10 |
2.273 |
2.247 |
2.260 |
2.110 |
| 5 |
1.762 |
1.845 |
1.804 |
1.654 |
| 2.5 |
1.156 |
1.214 |
1.185 |
1.035 |
| 1.25 |
0.687 |
0.674 |
0.681 |
0.531 |
| 0.625 |
0.356 |
0.369 |
0.363 |
0.213 |
| 0.312 |
0.258 |
0.239 |
0.249 |
0.099 |
| 0 |
0.147 |
0.152 |
0.150 |
|
|
| Materials provided |
- A micro ELISA plate --- The 96-well plate has been pre-coated with anti-rat IL-6 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard --- Dilute a bottle of standard at dilution series, read the OD values, and then draw a standard curve.
- Biotin-labeled IL-6 antibody (100 x concentrate) 1 x 120 μl ---Act as the detector antibody.
- HRP-avidin (100 x concentrate) 1 x 120 μl --- Avidin binds to the detection antibody, and HRP catalyzes the TMB substrate and thus changes the solution color.
- Biotin-antibody Diluent 1 x 15 ml ---Dilute the Biotin-antibody solution.
- HRP-avidin Diluent 1 x 15 ml ---Dilute the HRP-avidin solution.
- Sample Diluent 1 x 50 ml---Dilute the sample to an appropriate concentration.
- Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away unbound or free substances.
- TMB Substrate 1 x 10 ml --- The TMB-HRP coproduct appears blue after adding the chromogenic agent. After the enzyme reaction is terminated, the TMB product changes from blue to yellow. It can be quantified in the colorimeter.
- Stop Solution 1 x 10 ml --- Stop the color reaction.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
|
| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
