Rat Leukotriene B4,LT-B4 ELISA Kit
Product Details
| Target Name |
Leukotriene B4,LT-B4 |
| Alternative Names |
N/A |
| Abbreviation |
LT-B4 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
15.6 pg/mL-1000 pg/mL |
| Sensitivity |
3.9 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat LT-B4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
94 |
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| Range % |
89-99 |
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| 1:2 |
Average % |
97 |
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| Range % |
91-103 |
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| 1:4 |
Average % |
97 |
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| Range % |
92-104 |
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| 1:8 |
Average % |
93 |
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| Range % |
88-97 |
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| Recovery |
| The recovery of rat LT-B4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
87-96 |
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| EDTA plasma (n=4) |
97 |
93-106 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.442 |
2.541 |
2.492 |
2.320 |
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| 500 |
1.887 |
1.986 |
1.937 |
1.765 |
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| 250 |
1.282 |
1.263 |
1.273 |
1.101 |
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| 125 |
0.830 |
0.821 |
0.826 |
0.654 |
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| 62.5 |
0.511 |
0.502 |
0.507 |
0.335 |
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| 31.2 |
0.370 |
0.361 |
0.366 |
0.194 |
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| 15.6 |
0.264 |
0.259 |
0.262 |
0.090 |
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| 0 |
0.171 |
0.172 |
0.172 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
