Rat Low Density Lipoprotein cholesterol (LDL-C) ELISA kit
Product Details
| Target Name |
Low Density Lipoprotein cholesterol (LDL-C) |
| Abbreviation |
LDL-C |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
125 ng/mL-8000 ng/mL |
| Sensitivity |
62.5 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Competitive |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat LDL-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
98 |
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| Range % |
94-102 |
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| 1:200 |
Average % |
94 |
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| Range % |
91-97 |
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| 1:400 |
Average % |
102 |
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| Range % |
98-106 |
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| 1:800 |
Average % |
88 |
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| Range % |
84-92 |
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| Recovery |
| The recovery of rat LDL-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
89-99 |
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| EDTA plasma (n=4) |
103 |
98-109 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
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| 8000 |
0.089 |
0.088 |
0.089 |
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| 4000 |
0.182 |
0.178 |
0.180 |
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| 2000 |
0.376 |
0.369 |
0.373 |
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| 1000 |
0.630 |
0.642 |
0.636 |
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| 500 |
1.130 |
1.146 |
1.138 |
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| 250 |
1.528 |
1.516 |
1.522 |
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| 125 |
1.914 |
1.810 |
1.862 |
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| 0 |
2.267 |
2.169 |
2.218 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
