Rat Macrophage Colony-Stimulating Factor,M-CSF ELISA kit
Product Details
| Target Name |
colony stimulating factor 1 (macrophage) |
| Alternative Names |
Csf1 ELISA kit; CsfmMacrophage colony-stimulating factor 1 ELISA kit; CSF-1 ELISA kit; MCSF) [Cleaved into: Processed macrophage colony-stimulating factor 1] ELISA kit |
| Abbreviation |
CSF1 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
15.6 pg/mL-1000 pg/mL |
| Sensitivity |
3.9 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat M-CSF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
88 |
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| Range % |
82-95 |
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| 1:2 |
Average % |
98 |
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| Range % |
90-105 |
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| 1:4 |
Average % |
95 |
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| Range % |
90-100 |
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| 1:8 |
Average % |
90 |
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| Range % |
85-95 |
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| Recovery |
| The recovery of rat M-CSF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
98 |
92-104 |
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| EDTA plasma (n=4) |
92 |
87-97 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.413 |
2.352 |
2.383 |
2.192 |
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| 500 |
2.032 |
1.976 |
2.004 |
1.813 |
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| 250 |
1.658 |
1.543 |
1.601 |
1.410 |
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| 125 |
1.225 |
1.202 |
1.214 |
1.023 |
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| 62.5 |
0.869 |
0.824 |
0.847 |
0.656 |
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| 31.2 |
0.431 |
0.407 |
0.419 |
0.228 |
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| 15.6 |
0.341 |
0.315 |
0.328 |
0.137 |
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| 0 |
0.194 |
0.188 |
0.191 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
