Rat matrix metalloproteinase 13,MMP-13 ELISA kit
Product Details
| Target Name |
matrix metallopeptidase 13 (collagenase 3) |
| Alternative Names |
Mmp13 ELISA Kit; Collagenase 3 ELISA Kit; EC 3.4.24.- ELISA Kit; Matrix metalloproteinase-13 ELISA Kit; MMP-13 ELISA Kit; UMRCASE ELISA Kit; Fragment ELISA Kit |
| Abbreviation |
MMP13 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
93.75 pg/mL-6000 pg/mL |
| Sensitivity |
23.44 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
86 |
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| Range % |
83-90 |
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| 1:2 |
Average % |
94 |
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| Range % |
90-98 |
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| 1:4 |
Average % |
98 |
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| Range % |
93-104 |
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| 1:8 |
Average % |
102 |
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| Range % |
98-105 |
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| Recovery |
| The recovery of rat MMP-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-99 |
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| EDTA plasma (n=4) |
90 |
85-96 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 6000 |
2.231 |
2.269 |
2.250 |
2.138 |
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| 3000 |
1.526 |
1.539 |
1.533 |
1.421 |
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| 1500 |
0.974 |
0.997 |
0.986 |
0.874 |
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| 750 |
0.578 |
0.596 |
0.587 |
0.475 |
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| 375 |
0.345 |
0.327 |
0.336 |
0.224 |
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| 187.5 |
0.251 |
0.241 |
0.246 |
0.134 |
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| 93.75 |
0.185 |
0.189 |
0.187 |
0.075 |
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| 0 |
0.111 |
0.113 |
0.112 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
