Rat matrix metalloproteinase 3,MMP-3 ELISA kit
Product Details
| Target Name |
matrix metallopeptidase 3 (stromelysin 1, progelatinase) |
| Alternative Names |
Mmp3 ELISA Kit; Stromelysin-1 ELISA Kit; SL-1 ELISA Kit; EC 3.4.24.17 ELISA Kit; Matrix metalloproteinase-3 ELISA Kit; MMP-3 ELISA Kit; PTR1 protein ELISA Kit; Transin-1 ELISA Kit |
| Abbreviation |
MMP3 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
1.56 pg/mL-100 pg/mL |
| Sensitivity |
0.39 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
90 |
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| Range % |
87-93 |
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| 1:200 |
Average % |
94 |
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| Range % |
90-98 |
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| 1:400 |
Average % |
95 |
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| Range % |
91-99 |
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| 1:800 |
Average % |
92 |
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| Range % |
88-96 |
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| Recovery |
| The recovery of rat MMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
85 |
81-89 |
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| EDTA plasma (n=4) |
94 |
90-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 100 |
2.569 |
2.547 |
2.558 |
2.429 |
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| 50 |
2.236 |
2.213 |
2.225 |
2.096 |
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| 25 |
1.868 |
1.829 |
1.849 |
1.720 |
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| 12.5 |
1.367 |
1.387 |
1.377 |
1.248 |
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| 6.25 |
0.840 |
0.889 |
0.865 |
0.736 |
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| 3.13 |
0.377 |
0.387 |
0.382 |
0.253 |
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| 1.56 |
0.247 |
0.259 |
0.253 |
0.124 |
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| 0 |
0.128 |
0.130 |
0.129 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
