Rat myeloperoxidase-antineutrophil cytoplasmic antibody(MPO-ANCA) ELISA kit
Product Details
| Target Name |
myeloperoxidase-antineutrophil cytoplasmic antibody(MPO-ANCA) |
| Alternative Names |
N/A |
| Abbreviation |
MPO-ANCA |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma |
| Detection Range |
0.625 U/mL-40 U/mL |
| Sensitivity |
0.156 U/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat MPO-ANCA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
85 |
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| Range % |
80-95 |
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| 1:2 |
Average % |
97 |
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| Range % |
91-105 |
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| 1:4 |
Average % |
96 |
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| Range % |
92-100 |
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| 1:8 |
Average % |
93 |
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| Range % |
85-98 |
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| Recovery |
| The recovery of rat MPO-ANCA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
94 |
89-97 |
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| EDTA plasma (n=4) |
91 |
84-96 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| U/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 40 |
2.612 |
2.752 |
2.682 |
2.518 |
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| 20 |
2.013 |
2.076 |
2.045 |
1.881 |
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| 10 |
1.367 |
1.304 |
1.336 |
1.172 |
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| 5 |
0.802 |
0.837 |
0.820 |
0.656 |
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| 2.5 |
0.444 |
0.424 |
0.434 |
0.270 |
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| 1.25 |
0.351 |
0.359 |
0.355 |
0.191 |
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| 0.625 |
0.241 |
0.225 |
0.233 |
0.069 |
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| 0 |
0.160 |
0.167 |
0.164 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
