Rat N-terminal pro-brain natriuretic peptide,NT-proBNP ELISA KIT
Product Details
| Target Name |
N-terminal pro-brain natriuretic peptide,NT-proBNP |
| Abbreviation |
NT-proBNP |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
7.8 pg/mL-500 pg/mL |
| Sensitivity |
1.95 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat NT-proBNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
89 |
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| Range % |
84-95 |
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| 1:2 |
Average % |
96 |
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| Range % |
90-101 |
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| 1:4 |
Average % |
99 |
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| Range % |
94-105 |
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| 1:8 |
Average % |
84 |
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| Range % |
80-88 |
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| Recovery |
| The recovery of rat NT-proBNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
90 |
85-94 |
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| EDTA plasma (n=4) |
94 |
89-99 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 500 |
2.769 |
2.651 |
2.710 |
2.548 |
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| 250 |
1.973 |
1.901 |
1.937 |
1.775 |
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| 125 |
1.183 |
1.217 |
1.200 |
1.038 |
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| 62.5 |
0.793 |
0.841 |
0.817 |
0.655 |
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| 31.2 |
0.506 |
0.550 |
0.528 |
0.366 |
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| 15.6 |
0.404 |
0.379 |
0.392 |
0.230 |
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| 7.8 |
0.261 |
0.245 |
0.253 |
0.091 |
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| 0 |
0.165 |
0.159 |
0.162 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
