| Target Name |
Nesfatin-1 |
| Abbreviation |
Nesfatin-1 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
15.6 pg/mL-1000 pg/mL |
| Sensitivity |
3.9 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat nesfatin-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
84 |
|
| Range % |
80-92 |
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| 1:2 |
Average % |
89 |
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| Range % |
85-95 |
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| 1:4 |
Average % |
93 |
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| Range % |
89-99 |
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| 1:8 |
Average % |
95 |
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| Range % |
90-100 |
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| Recovery |
| The recovery of rat nesfatin-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
104 |
97-109 |
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| EDTA plasma (n=4) |
97 |
93-102 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 1000 |
2.795 |
2.652 |
2.724 |
2.573 |
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| 500 |
1.857 |
1.776 |
1.817 |
1.666 |
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| 250 |
1.004 |
1.082 |
1.043 |
0.892 |
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| 125 |
0.654 |
0.613 |
0.634 |
0.483 |
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| 62.5 |
0.424 |
0.430 |
0.427 |
0.276 |
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| 31.2 |
0.317 |
0.299 |
0.308 |
0.157 |
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| 15.6 |
0.226 |
0.209 |
0.218 |
0.067 |
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| 0 |
0.154 |
0.147 |
0.151 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
