Rat Neurotrophin 3,NT-3 ELISA KIT
Product Details
| Target Name |
neurotrophin 3 |
| Alternative Names |
Ntf3 ELISA Kit; Ntf-3 ELISA Kit; Neurotrophin-3 ELISA Kit; NT-3 ELISA Kit; HDNF ELISA Kit; Nerve growth factor 2 ELISA Kit; NGF-2 ELISA Kit; Neurotrophic factor ELISA Kit |
| Abbreviation |
NTF3 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.9 ng/mL-60 ng/mL |
| Sensitivity |
0.225 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Neuroscience |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat NT-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
88-96 |
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| 1:2 |
Average % |
94 |
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| Range % |
89-98 |
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| 1:4 |
Average % |
97 |
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| Range % |
93-101 |
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| 1:8 |
Average % |
95 |
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| Range % |
89-98 |
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| Recovery |
| The recovery of rat NT-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
88 |
82-94 |
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| EDTA plasma (n=4) |
92 |
86-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 60 |
2.512 |
2.619 |
2.566 |
2.434 |
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| 30 |
2.235 |
2.121 |
2.178 |
2.046 |
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| 15 |
1.526 |
1.504 |
1.515 |
1.383 |
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| 7.5 |
0.812 |
0.823 |
0.818 |
0.686 |
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| 3.75 |
0.458 |
0.446 |
0.452 |
0.320 |
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| 1.8 |
0.326 |
0.314 |
0.320 |
0.188 |
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| 0.9 |
0.221 |
0.227 |
0.224 |
0.092 |
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| 0 |
0.133 |
0.131 |
0.132 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
