Rat Neurotrophin 4,NT-4 ELISA KIT
Product Details
| Target Name |
neurotrophin 4 |
| Alternative Names |
Ntf4 ELISA Kit; Nt4 ELISA Kit; Ntf5 ELISA Kit; Neurotrophin-4 ELISA Kit; NT-4 ELISA Kit; Neurotrophin-5 ELISA Kit; NT-5 ELISA Kit; Neutrophic factor 4 ELISA Kit |
| Abbreviation |
NTF4 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
47 pg/mL-3000 pg/mL |
| Sensitivity |
11.75 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Neuroscience |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat NT-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
86 |
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| Range % |
82-94 |
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| 1:2 |
Average % |
93 |
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| Range % |
88-98 |
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| 1:4 |
Average % |
96 |
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| Range % |
90-102 |
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| 1:8 |
Average % |
99 |
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| Range % |
95-104 |
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| Recovery |
| The recovery of rat NT-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
96 |
90-102 |
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| EDTA plasma (n=4) |
100 |
95-105 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 3000 |
2.268 |
2.250 |
2.259 |
2.150 |
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| 1500 |
1.845 |
1.871 |
1.858 |
1.749 |
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| 750 |
1.464 |
1.492 |
1.478 |
1.369 |
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| 375 |
1.135 |
1.149 |
1.142 |
1.033 |
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| 187.5 |
0.704 |
0.724 |
0.714 |
0.605 |
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| 94 |
0.438 |
0.457 |
0.448 |
0.339 |
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| 47 |
0.287 |
0.294 |
0.291 |
0.182 |
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| 0 |
0.108 |
0.110 |
0.109 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
